描述
Taq DNA Polymerase is a simple, specificity PCR reaction mixture. Simply add primers, template, dNTPs, and PCR grade water, the reagent will execute primer extensions and other molecular biology applications. Taq DNA Polymerase contains two components, include the Taq DNA polymerase and 10X PCR buffer. The Taq DNA Polymerase is purified from E coli., expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ →3′ DNA polymerase and a 5′ → 3′ exonuclease activity but lacks a 3′ → 5′ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kilo Dalton. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from
single-stranded templates in the presence of a primer. TheTaq DNA Polymerase is recommended for use in routine PCR reactions. The 10X PCR buffer is optimized for high specificity and guarantees minimal by product formation. Usually 1-1.5 unit of Taq DNA polymerase is used in 50 μl of reaction mix. Higher Taq DNA polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors exist in the reaction mixture (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA polymerase (2-3 units) may be necessary to obtain a better yield of amplification products.
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